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Electron microscopy / Image analysis / 3D reconstruction

TrakEM2

  • TrakEM2

Exporting ROI-substacks from TrakEM2 with known coordinate positions

Posted on 20. July 201930. June 2022by Stefan

There are two ways to extract the edge coordinates of an region of interest (ROI): 1) The long wayBefore starting to draw the region of interest (rectangle tool) and exporting the images (export –> make flat image), we start the macro recorder (FIJI Side bar: Plugins –> Macro –> Record), which allows to read out […]

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  • Amira
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    • TrakEM2

Import of large Amira label files to TrakEM2

Posted on 11. July 201930. June 2022by Stefan

Some projects might need in a later project step a realignment of the image data (after segmentations have been done already) to correct for so far overlooked smaller alignment errors. It is possible to recalculate the Amira labels to the new image transformations in TrakEM2 (assumed you have done the initial image alignment in TrakEM2), […]

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About me

Hi, I am Stefan Fischer. I am biologist at the University of Tübingen and Head of Tübingen Structural Microscopy Core Facility (TSM). My methodological interest is set on electron microscopy. Furthermore I do focus on 3D techniques, be it on basis of semi-thin sections (LM), Ultra-thin sections (ssTEM) and also MicroCT data. For that reason I developed a strong interest in open source software packages and image analysis in order to automate (or semi-automate) some of the tedious parts of my workflows.

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new posts

  • Converting MicroCT images from 32bit to 16bit or 8bit in FIJI
  • Amira: Error
  • Exporting ROI-substacks from TrakEM2 with known coordinate positions
  • Extract image data below labels in Amira
  • Extracting specific labels from label fields in Amira

keywords

alignment Amira Arithmetic tool commandline exchange export Fiji Import Label macro memory MicroCT preprocessing ROI selection SEM terminal Trak TrakEM2 ultramicrotomy

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